cellular localization meaning in Chinese
细胞定位
Examples
- The cellular localization of hse was done by using in situ hybridization , the results showed that primary spermtatocytes and spermatids in seminiferous tubular have positive signals . the subcellular localizations of hsei and hseii were identified by gfp fusion protein . hsei - gfp fusion protein was evenly distributing in cytoplasm , while hseii - gfp was not evenly distributing in cytoplasm and there were many bright spots in cytosol
用gfp高合蛋白的技术确定hsei和hsell在细胞中勺定位，结果显示hsei gfp均匀地分布于细胞浆中，而hsell0fp则分布不均匀，在胞浆有聚集成许多荧光信号很强的点。 - The cellular localization of kvl . 2 and the co - localization of kvl . 2 and vegf receptors in the rat brain in rat cerebral cortex , triple - fluorescence labeling for kvl . 2 , nse , and gfap showed that kvl . 2 immunopositive labeling was predominantly seen on the membrane of the cells co - stained with nse , a specific neuronal marker . only few kvl . 2 positive labeling cells were co - stained with gfap , an astrocyte glial cell marker
大鼠脑内kv1 . 2蛋白的细胞定位及其与vegf受体间的共存关系大鼠大脑皮层kv1 . 2 、 nse 、 gfap免疫荧光三标结果： kv1 . 2蛋白分布在细胞膜上，大部分kv1 . 2免疫阳性细胞表达有nse ，只有在少量kv1 . 2阳性细胞上表达有gfap 。 - To make clear the hypothesis , a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study , respectively . we first studied the cellular localization of kvl . 2 and the co - localization of kvl . 2 protein and vegf receptors flk - 1 and flt - 1 , observed the effect of mcao on kvl . 2 expression and phosphrylation in the rat brain in vivo , then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl . 2 in sh - sy5y cells . finally , in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl . 2 phosphorylation , we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl . 2 phosphorylation
为了验证上述假设，本文分别在整体和离体水平，采用大脑中动脉缺血( middlecerebralarteryocclusion ， mcao )和体外氧?糖剥夺( hypoxiaandglucose - deprivation ， hgd )缺血模型，首先了解了kv1 . 2蛋白的细胞定位及与vegf受体flk - 1和flt - 1的共存情况，观察了整体mcao后缺血再灌不同时间大鼠脑内kv1 . 2蛋白的磷酸化水平变化，然后通过外源性给予vegf蛋白，在sh - sy5y细胞株上观察其对缺血细胞存活率及kv1 . 2蛋白磷酸化水平的影响，最后利用vegf反义脱氧寡核苷酸( oligodeoxynucleotide ， odn )特异阻断内源性vegf蛋白的表达，观察内源性vegf蛋白在缺血细胞损伤及调节kv1 . 2蛋白磷酸化中的作用，以进一步明确vegf缺血保护效应与其调节kv1 . 2蛋白磷酸化之间的关系。 - In this study , we showed that the pdk inhibitor ly294002 regulated the cellular localization of materials 1 . kunming mice were supplied from the department of laboratory animals , china medical university 2 . pregnant mare serum gonadotropin ( pmsg ) and human chorionic gona - dotropin ( hcg ) were obtained from tianjin huafu biological products research institute and shanghai products research insititute , respectively
为了研究在小鼠受精卵中pkc 、 pkb是否也通过p21蛋白参与调控g2 m转换，本实验应用了pma和ly294002作为pkc 、 pkb的抑制剂，研究它们对小鼠受精卵中p21蛋白表达和定位，进一步探讨可能的调控机制。 - We fused the gfp into the c - terminal region as well as n - terminal region of emt - 1 protein . the result showed that the localization of the protein encoded by the full length emt - 1 cdna was in endoplastic reticulum ( er ) . extracellular region , transmembrane and intracellular region showed the similar cellular localization
我们用绿色荧光蛋白融合于emt l全长及其不同截断形式的梭基和氨基端，瞬时转染cos 7细胞，通过荧光显微镜观察，发现emt l编码的蛋白呈现内质网定位的特点。